Description

Biomeme’s Dengue Virus Subtyping RT-PCR Test is intended to detect and differentiates the RNA of the 4 major Dengue Virus (DENV) serotypes. Dengue virus is a mosquito-borne single positive-stranded RNA virus of the family Flaviviridae; genus Flavivirus. Each test already contains lyophilized master mix, enzymes, and multiplexed primers/probes for the following triplex reaction:

Layout

Rxn Well	Green	Amber	Red
1	DENV-2	MS2	DENV-1
2	DENV-3	N/A	N/A
3	N/A	RNase P	DENV-4

Chemistry

Rxn Well	Green	Amber	Red
1	FAM	TxRedX	QUASAR670
2	FAM	N/A	N/A
3	N/A	TxRedX-XN	Cy5

Go-Strips™ are pre-aliquoted, lyophilized strips optimized for outstanding performance in the field with no need for refrigeration, lab equipment, or mixing of wet reagents. They are designed to work with Biomeme's ultra-portable Franklin™ thermocyclers.

Each order includes the following:

Item	Description	Quantity
Dengue Virus Subtyping 3-Well Go-Strips™	Each well of each strip contains a 20µL lyophilized triplex reaction with master mix, primers and probes.	32 (96 rxns)
3-Well Void Filling Caps	Each cap is used to seal your PCR reactions inside your Go-Strip™ before placing into thermocycler.	32

Highlights

A multiplexed reaction means that all targets are in a single reaction. So, a single reaction equates to a single sample.
Because each test is lyophilized, you are able to add more sample (“template”) to the test (20 µL) which will directly increase sensitivity without throwing off your reagent concentrations.
Most other assays require shipment on dry ice and storage in a -80°C freezer which can increase the risk of components going bad due to freeze and thaw cycles. With our test, there is no need for refrigeration, lab equipment, or mixing of wet reagents.
Most assays come in multiple components or in bulk and need to be mixed and aliquoted by an experienced molecular biologist, further increasing the risk of contamination. There is no mixing or aliquoting of components required with our test. It’s already taken care of.