Biomeme’s BioPoo® E. coli RT-PCR Go-Strips® Test detects and differntiates Escherichia coli and fecal indicator bacteria DNA and already contains lyophilized master mix, enzymes, and multiplexed primers/probes for the following triplex reaction:
|Green (FAM)||Amber (TexRedX)||Red (ATTO647N)|
|E. coli||Human fecal bacterial indicator||Internal Positive Control (IPC)|
Go-Strips® are pre-aliquoted, lyophilized strips optimized for outstanding performance in the field with no need for refrigeration, lab equipment, or mixing of wet reagents. They are designed to work with Biomeme's ultra-portable Franklin™ thermocyclers.
Each order includes the following:
|BioPoo® E. coli 3-Well Go-Strips®||Each well of each strip contains a 20µL lyophilized triplex reaction with master mix, primers and probes.||32 (96 rxns)|
|3-Well Void Filling Caps||Each cap is used to seal your PCR reactions inside your Go-Strip™ before placing into thermocycler.||32|
A multiplexed reaction means that all targets are in a single reaction. So, a single reaction equates to a single sample.
Because each test is lyophilized, you are able to add more sample (“template”) to the test (20 µL) which will directly increase sensitivity without throwing off your reagent concentrations.
Most other assays require shipment on dry ice and storage in a -80°C freezer which can increase the risk of components going bad due to freeze and thaw cycles. With our test, there is no need for refrigeration, lab equipment, or mixing of wet reagents.
Most assays come in multiple components or in bulk and need to be mixed and aliquoted by an experienced molecular biologist, further increasing the risk of contamination. There is no mixing or aliquoting of components required with our test. It’s already taken care of.
The EPA recommends using the criteria in Table 1 when making a risk management decision recreational water quality. For salt water, EPA recommends testing for Enterococcus faecalis.
For Research Use Only (RUO). Not for use in human or veterinary diagnostics.
If you have any other questions, check out help.biomeme.com to learn more.